RT Journal
A1 Bruno, Vincent M.
A1 Wang, Zhong
A1 Marjani, Sadie L.
A1 Euskirchen, Ghia M.
A1 Martin, Jeffrey
A1 Sherlock, Gavin
A1 Snyder, Michael
T1 Comprehensive annotation of the transcriptome of the human fungal pathogen Candida albicans using RNA-seq
JF Genome Research 
JO Genome Research 
YR 2010 
FD October 01 
VO 20 
IS 10 
SP 1451 
OP 1458 
DO 10.1101/gr.109553.110 
UL http://genome.cshlp.org/content/20/10/1451.abstract 
AB 
Candida albicans is the major invasive fungal pathogen of humans, causing diseases ranging from superficial mucosal infections to disseminated, systemic infections that are often lifethreatening. We have used massively parallel high-throughput sequencing of cDNA (RNA-seq) to generate a high-resolution map of the C. albicans transcriptome under several different environmental conditions. We have quantitatively determined all of the regions that are transcribed under these different conditions, and have identified 602 novel transcriptionally active regions (TARs) and numerous novel introns that are not represented in the current genome annotation. Interestingly, the expression of many of these TARs is regulated in a condition-specific manner. This comprehensive transcriptome analysis significantly enhances the current genome annotation of C. albicans, a necessary framework for a complete understanding of the molecular mechanisms of pathogenesis for this important eukaryotic pathogen.