TY  - JOUR
A1  - Andersson, B
A1  - Ying, J H
A1  - Lewis, D E
A1  - Gibbs, R A
T1  - Rapid characterization of HIV-1 sequence diversity using denaturing gradient gel electrophoresis and direct automated DNA sequencing of PCR products.
Y1  - 1993/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 293 
EP  - 300 
DO  - 10.1101/gr.2.4.293 
VL  - 2 
IS  - 4 
UR  - http://genome.cshlp.org/content/2/4/293.abstract 
N2  - A direct method for visualization and isolation of sequence variants of human immunodeficiency virus type 1 (HIV-1) utilizing denaturing gradient gel electrophoresis (DGGE) combined with automated direct DNA sequencing was developed. Two fragments from the env gene and one from the nef gene of HIV-1, which together constitute approximately 1.0 kb of sequence, were amplified by PCR and analyzed. HIV-1 variants from each region were resolved and excised from the gel; this was followed by direct sequencing of different viral variants. In 9 infected patients, a limited number of dominant sequence variants could be seen in the three regions, together with a faint background of minor variants. The use of DGGE makes it possible to obtain a direct estimate of overall HIV-1 sequence diversity within patient samples without an intermediate DNA cloning step. 
ER  -