@article{Andersson01051993,
author = {Andersson, B and Ying, J H and Lewis, D E and Gibbs, R A}, 
title = {Rapid characterization of HIV-1 sequence diversity using denaturing gradient gel electrophoresis and direct automated DNA sequencing of PCR products.},
volume = {2}, 
number = {4}, 
pages = {293-300}, 
year = {1993}, 
doi = {10.1101/gr.2.4.293}, 
abstract ={A direct method for visualization and isolation of sequence variants of human immunodeficiency virus type 1 (HIV-1) utilizing denaturing gradient gel electrophoresis (DGGE) combined with automated direct DNA sequencing was developed. Two fragments from the env gene and one from the nef gene of HIV-1, which together constitute approximately 1.0 kb of sequence, were amplified by PCR and analyzed. HIV-1 variants from each region were resolved and excised from the gel; this was followed by direct sequencing of different viral variants. In 9 infected patients, a limited number of dominant sequence variants could be seen in the three regions, together with a faint background of minor variants. The use of DGGE makes it possible to obtain a direct estimate of overall HIV-1 sequence diversity within patient samples without an intermediate DNA cloning step.}, 
URL = {http://genome.cshlp.org/content/2/4/293.abstract}, 
eprint = {http://genome.cshlp.org/content/2/4/293.full.pdf+html}, 
journal = {Genome Research} 
}