RT Journal
A1 Giusti, W G
A1 Adriano, T
T1 Synthesis and characterization of 5'-fluorescent-dye-labeled oligonucleotides.
JF Genome Research 
JO Genome Research 
YR 1993 
FD February 01 
VO 2 
IS 3 
SP 223 
OP 227 
DO 10.1101/gr.2.3.223 
UL http://genome.cshlp.org/content/2/3/223.abstract 
AB Fluorescent-dye-labeled oligonucleotides are used in many procedures, including DNA sequencing, PCR, restriction mapping, the study of genetic disease, and forensics identification. In this paper, we describe detailed methods for the synthesis, purification, and quantification of 5'-fluorescent-dye-labeled oligonucleotides. The relationship of specific and nonspecific dye attachment to synthetic oligonucleotides is discussed, and the importance of primer design is considered as a way to prevent chemical problems from interfering with biological reactions.