TY  - JOUR
A1  - Coriat, A M
A1  - Müller, U
A1  - Harry, J L
A1  - Uwanogho, D
A1  - Sharpe, P T
T1  - PCR amplification of SRY-related gene sequences reveals evolutionary conservation of the SRY-box motif.
Y1  - 1993/02/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 218 
EP  - 222 
DO  - 10.1101/gr.2.3.218 
VL  - 2 
IS  - 3 
UR  - http://genome.cshlp.org/content/2/3/218.abstract 
N2  - SRY (sex-determining region of the Y chromosome) has recently been identified as a key regulatory gene in mammalian sex determination. The open reading frame of this gene contains an 80-amino-acid motif, the SRY-box, which shares a high degree of homology with a DNA-binding domain found in the high-mobility-group (HMG) proteins HMG1 and HMG2. The SRY box motif is highly conserved in several sequence-specific DNA-binding proteins that are known to act as transcription factors. Here we describe the use of degenerate PCR primers to identify SRY-related sequences containing the SRY-box motif from the genomic DNA of a variety of species. The results of this study suggest that in a diverse array of species SRY-related genes may serve as transcription factors that regulate a variety of developmental pathways, including sex determination. 
ER  -