RT Journal
A1 Jones, D H
A1 Winistorfer, S C
T1 Genome walking with 2- to 4-kb steps using panhandle PCR.
JF Genome Research 
JO Genome Research 
YR 1993 
FD February 01 
VO 2 
IS 3 
SP 197 
OP 203 
DO 10.1101/gr.2.3.197 
UL http://genome.cshlp.org/content/2/3/197.abstract 
AB Panhandle PCR is a method that permits the rapid retrieval of DNA segments flanking a known DNA sequence. This method extends the application of PCR to the retrieval of DNA where only one end of the DNA sequence is known, so that one can use PCR to walk along an uncharacterized stretch of DNA. In this report, we demonstrate that this method can be applied to the highly specific retrieval of large pieces (2.2-4.4 kb) of human genomic DNA that flank the primer annealing sites.