TY  - JOUR
A1  - Lucas, W T
A1  - Youngner, J S
T1  - The use of hybrid-selected template increases the specificity of the polymerase chain reaction.
Y1  - 1992/08/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 41 
EP  - 44 
DO  - 10.1101/gr.2.1.41 
VL  - 2 
IS  - 1 
UR  - http://genome.cshlp.org/content/2/1/41.abstract 
N2  - An efficient method for generating full-length DNA sequences from apparently unsuccessful polymerase chain reactions (PCR) has been developed. In cases where nonspecific background interferes with detection of the PCR product, a second amplification is performed using a nested set of primers. The internal fragment of DNA amplified in this reaction is then blotted to a membrane and used to hybrid-select the desired DNA from the initial amplification. This DNA is eluted and used as the template for a third round of PCR. The re-use of the original primers from the initial reaction enables the final PCR to generate full-length DNA. This technique was used to clone a full-length gene segment 8 from a mutant influenza A/WSN/33 (H1N1) virus after initial PCR attempts had failed. 
ER  -