@article{Xin01072009,
author = {Xin, Xiaofeng and Rual, Jean-François and Hirozane-Kishikawa, Tomoko and Hill, David E. and Vidal, Marc and Boone, Charles and Thierry-Mieg, Nicolas}, 
title = {Shifted Transversal Design smart-pooling for high coverage interactome mapping},
volume = {19}, 
number = {7}, 
pages = {1262-1269}, 
year = {2009}, 
doi = {10.1101/gr.090019.108}, 
abstract ={“Smart-pooling,” in which test reagents are multiplexed in a highly redundant manner, is a promising strategy for achieving high efficiency, sensitivity, and specificity in systems-level projects. However, previous applications relied on low redundancy designs that do not leverage the full potential of smart-pooling, and more powerful theoretical constructions, such as the Shifted Transversal Design (STD), lack experimental validation. Here we evaluate STD smart-pooling in yeast two-hybrid (Y2H) interactome mapping. We employed two STD designs and two established methods to perform ORFeome-wide Y2H screens with 12 baits. We found that STD pooling achieves similar levels of sensitivity and specificity as one-on-one array-based Y2H, while the costs and workloads are divided by three. The screening-sequencing approach is the most cost- and labor-efficient, yet STD identifies about twofold more interactions. Screening-sequencing remains an appropriate method for quickly producing low-coverage interactomes, while STD pooling appears as the method of choice for obtaining maps with higher coverage.}, 
URL = {http://genome.cshlp.org/content/19/7/1262.abstract}, 
eprint = {http://genome.cshlp.org/content/19/7/1262.full.pdf+html}, 
journal = {Genome Research} 
}