RT Journal
A1 Lister, Ryan
A1 Ecker, Joseph R.
T1 Finding the fifth base: Genome-wide sequencing of cytosine methylation
JF Genome Research 
JO Genome Research 
YR 2009 
FD June 01 
VO 19 
IS 6 
SP 959 
OP 966 
DO 10.1101/gr.083451.108 
UL http://genome.cshlp.org/content/19/6/959.abstract 
AB Complete sequences of myriad eukaryotic genomes, including several human genomes, are now available, and recent dramatic developments in DNA sequencing technology are opening the floodgates to vast volumes of sequence data. Yet, despite knowing for several decades that a significant proportion of cytosines in the genomes of plants and animals are present in the form of methylcytosine, until very recently the precise locations of these modified bases have never been accurately mapped throughout a eukaryotic genome. Advanced “next-generation” DNA sequencing technologies are now enabling the global mapping of this epigenetic modification at single-base resolution, providing new insights into the regulation and dynamics of DNA methylation in genomes.