TY - JOUR A1 - The MGC Project Team A1 - Temple, Gary A1 - Gerhard, Daniela S. A1 - Rasooly, Rebekah A1 - Feingold, Elise A. A1 - Good, Peter J. A1 - Robinson, Cristen A1 - Mandich, Allison A1 - Derge, Jeffrey G. A1 - Lewis, Jeanne A1 - Shoaf, Debonny A1 - Collins, Francis S. A1 - Jang, Wonhee A1 - Wagner, Lukas A1 - Shenmen, Carolyn M. A1 - Misquitta, Leonie A1 - Schaefer, Carl F. A1 - Buetow, Kenneth H. A1 - Bonner, Tom I. A1 - Yankie, Linda A1 - Ward, Ming A1 - Phan, Lon A1 - Astashyn, Alex A1 - Brown, Garth A1 - Farrell, Catherine A1 - Hart, Jennifer A1 - Landrum, Melissa A1 - Maidak, Bonnie L. A1 - Murphy, Michael A1 - Murphy, Terence A1 - Rajput, Bhanu A1 - Riddick, Lillian A1 - Webb, David A1 - Weber, Janet A1 - Wu, Wendy A1 - Pruitt, Kim D. A1 - Maglott, Donna A1 - Siepel, Adam A1 - Brejova, Brona A1 - Diekhans, Mark A1 - Harte, Rachel A1 - Baertsch, Robert A1 - Kent, Jim A1 - Haussler, David A1 - Brent, Michael A1 - Langton, Laura A1 - Comstock, Charles L.G. A1 - Stevens, Michael A1 - Wei, Chaochun A1 - van Baren, Marijke J. A1 - Salehi-Ashtiani, Kourosh A1 - Murray, Ryan R. A1 - Ghamsari, Lila A1 - Mello, Elizabeth A1 - Lin, Chenwei A1 - Pennacchio, Christa A1 - Schreiber, Kirsten A1 - Shapiro, Nicole A1 - Marsh, Amber A1 - Pardes, Elizabeth A1 - Moore, Troy A1 - Lebeau, Anita A1 - Muratet, Mike A1 - Simmons, Blake A1 - Kloske, David A1 - Sieja, Stephanie A1 - Hudson, James A1 - Sethupathy, Praveen A1 - Brownstein, Michael A1 - Bhat, Narayan A1 - Lazar, Joseph A1 - Jacob, Howard A1 - Gruber, Chris E. A1 - Smith, Mark R. A1 - McPherson, John A1 - Garcia, Angela M. A1 - Gunaratne, Preethi H. A1 - Wu, Jiaqian A1 - Muzny, Donna A1 - Gibbs, Richard A. A1 - Young, Alice C. A1 - Bouffard, Gerard G. A1 - Blakesley, Robert W. A1 - Mullikin, Jim A1 - Green, Eric D. A1 - Dickson, Mark C. A1 - Rodriguez, Alex C. A1 - Grimwood, Jane A1 - Schmutz, Jeremy A1 - Myers, Richard M. A1 - Hirst, Martin A1 - Zeng, Thomas A1 - Tse, Kane A1 - Moksa, Michelle A1 - Deng, Merinda A1 - Ma, Kevin A1 - Mah, Diana A1 - Pang, Johnson A1 - Taylor, Greg A1 - Chuah, Eric A1 - Deng, Athena A1 - Fichter, Keith A1 - Go, Anne A1 - Lee, Stephanie A1 - Wang, Jing A1 - Griffith, Malachi A1 - Morin, Ryan A1 - Moore, Richard A. A1 - Mayo, Michael A1 - Munro, Sarah A1 - Wagner, Susan A1 - Jones, Steven J.M. A1 - Holt, Robert A. A1 - Marra, Marco A. A1 - Lu, Sun A1 - Yang, Shuwei A1 - Hartigan, James A1 - Graf, Marcus A1 - Wagner, Ralf A1 - Letovksy, Stanley A1 - Pulido, Jacqueline C. A1 - Robison, Keith A1 - Esposito, Dominic A1 - Hartley, James A1 - Wall, Vanessa E. A1 - Hopkins, Ralph F. A1 - Ohara, Osamu A1 - Wiemann, Stefan T1 - The completion of the Mammalian Gene Collection (MGC) Y1 - 2009/12/01 JF - Genome Research JO - Genome Research SP - 2324 EP - 2333 DO - 10.1101/gr.095976.109 VL - 19 IS - 12 UR - http://genome.cshlp.org/content/19/12/2324.abstract N2 - Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide. ER -