TY  - JOUR
A1  - Stambuk, Boris U.
A1  - Dunn, Barbara
A1  - Alves, Sergio L.
A1  - Duval, Eduarda H.
A1  - Sherlock, Gavin
T1  - Industrial fuel ethanol yeasts contain adaptive copy number changes in genes involved in vitamin B1 and B6 biosynthesis
Y1  - 2009/12/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2271 
EP  - 2278 
DO  - 10.1101/gr.094276.109 
VL  - 19 
IS  - 12 
UR  - http://genome.cshlp.org/content/19/12/2271.abstract 
N2  - Fuel ethanol is now a global energy commodity that is competitive with gasoline. Using microarray-based comparative genome hybridization (aCGH), we have determined gene copy number variations (CNVs) common to five industrially important fuel ethanol Saccharomyces cerevisiae strains responsible for the production of billions of gallons of fuel ethanol per year from sugarcane. These strains have significant amplifications of the telomeric SNO and SNZ genes, which are involved in the biosynthesis of vitamins B6 (pyridoxine) and B1 (thiamin). We show that increased copy number of these genes confers the ability to grow more efficiently under the repressing effects of thiamin, especially in medium lacking pyridoxine and with high sugar concentrations. These genetic changes have likely been adaptive and selected for in the industrial environment, and may be required for the efficient utilization of biomass-derived sugars from other renewable feedstocks. 
ER  -