RT Journal
A1 Kosakovsky Pond, Sergei
A1 Wadhawan, Samir
A1 Chiaromonte, Francesca
A1 Ananda, Guruprasad
A1 Chung, Wen-Yu
A1 Taylor, James
A1 Nekrutenko, Anton
A1 The Galaxy Team
T1 Windshield splatter analysis with the Galaxy metagenomic pipeline
JF Genome Research 
JO Genome Research 
YR 2009 
FD November 01 
VO 19 
IS 11 
SP 2144 
OP 2153 
DO 10.1101/gr.094508.109 
UL http://genome.cshlp.org/content/19/11/2144.abstract 
AB How many species inhabit our immediate surroundings? A straightforward collection technique suitable for answering this question is known to anyone who has ever driven a car at highway speeds. The windshield of a moving vehicle is subjected to numerous insect strikes and can be used as a collection device for representative sampling. Unfortunately the analysis of biological material collected in that manner, as with most metagenomic studies, proves to be rather demanding due to the large number of required tools and considerable computational infrastructure. In this study, we use organic matter collected by a moving vehicle to design and test a comprehensive pipeline for phylogenetic profiling of metagenomic samples that includes all steps from processing and quality control of data generated by next-generation sequencing technologies to statistical analyses and data visualization. To the best of our knowledge, this is also the first publication that features a live online supplement providing access to exact analyses and workflows used in the article.