TY  - JOUR
A1  - Kosakovsky Pond, Sergei
A1  - Wadhawan, Samir
A1  - Chiaromonte, Francesca
A1  - Ananda, Guruprasad
A1  - Chung, Wen-Yu
A1  - Taylor, James
A1  - Nekrutenko, Anton
A1  - The Galaxy Team
T1  - Windshield splatter analysis with the Galaxy metagenomic pipeline
Y1  - 2009/11/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2144 
EP  - 2153 
DO  - 10.1101/gr.094508.109 
VL  - 19 
IS  - 11 
UR  - http://genome.cshlp.org/content/19/11/2144.abstract 
N2  - How many species inhabit our immediate surroundings? A straightforward collection technique suitable for answering this question is known to anyone who has ever driven a car at highway speeds. The windshield of a moving vehicle is subjected to numerous insect strikes and can be used as a collection device for representative sampling. Unfortunately the analysis of biological material collected in that manner, as with most metagenomic studies, proves to be rather demanding due to the large number of required tools and considerable computational infrastructure. In this study, we use organic matter collected by a moving vehicle to design and test a comprehensive pipeline for phylogenetic profiling of metagenomic samples that includes all steps from processing and quality control of data generated by next-generation sequencing technologies to statistical analyses and data visualization. To the best of our knowledge, this is also the first publication that features a live online supplement providing access to exact analyses and workflows used in the article. 
ER  -