TY  - JOUR
A1  - Stinear, Timothy P.
A1  - Seemann, Torsten
A1  - Harrison, Paul F.
A1  - Jenkin, Grant A.
A1  - Davies, John K.
A1  - Johnson, Paul D.R.
A1  - Abdellah, Zahra
A1  - Arrowsmith, Claire
A1  - Chillingworth, Tracey
A1  - Churcher, Carol
A1  - Clarke, Kay
A1  - Cronin, Ann
A1  - Davis, Paul
A1  - Goodhead, Ian
A1  - Holroyd, Nancy
A1  - Jagels, Kay
A1  - Lord, Angela
A1  - Moule, Sharon
A1  - Mungall, Karen
A1  - Norbertczak, Halina
A1  - Quail, Michael A.
A1  - Rabbinowitsch, Ester
A1  - Walker, Danielle
A1  - White, Brian
A1  - Whitehead, Sally
A1  - Small, Pamela L.C.
A1  - Brosch, Roland
A1  - Ramakrishnan, Lalita
A1  - Fischbach, Michael A.
A1  - Parkhill, Julian
A1  - Cole, Stewart T.
T1  - Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis
Y1  - 2008/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 729 
EP  - 741 
DO  - 10.1101/gr.075069.107 
VL  - 18 
IS  - 5 
UR  - http://genome.cshlp.org/content/18/5/729.abstract 
N2  - Mycobacterium marinum, a ubiquitous pathogen of fish and amphibia, is a near relative of Mycobacterium tuberculosis, the etiologic agent of tuberculosis in humans. The genome of the M strain of M. marinum comprises a 6,636,827-bp circular chromosome with 5424 CDS, 10 prophages, and a 23-kb mercury-resistance plasmid. Prominent features are the very large number of genes (57) encoding polyketide synthases (PKSs) and nonribosomal peptide synthases (NRPSs) and the most extensive repertoire yet reported of the mycobacteria-restricted PE and PPE proteins, and related-ESX secretion systems. Some of the NRPS genes comprise a novel family and seem to have been acquired horizontally. M. marinum is used widely as a model organism to study M. tuberculosis pathogenesis, and genome comparisons confirmed the close genetic relationship between these two species, as they share 3000 orthologs with an average amino acid identity of 85%. Comparisons with the more distantly related Mycobacterium avium subspecies paratuberculosis and Mycobacterium smegmatis reveal how an ancestral generalist mycobacterium evolved into M. tuberculosis and M. marinum. M. tuberculosis has undergone genome downsizing and extensive lateral gene transfer to become a specialized pathogen of humans and other primates without retaining an environmental niche. M. marinum has maintained a large genome so as to retain the capacity for environmental survival while becoming a broad host range pathogen that produces disease strikingly similar to M. tuberculosis. The work described herein provides a foundation for using M. marinum to better understand the determinants of pathogenesis of tuberculosis. 
ER  -