@article{Stinear01052008,
author = {Stinear, Timothy P. and Seemann, Torsten and Harrison, Paul F. and Jenkin, Grant A. and Davies, John K. and Johnson, Paul D.R. and Abdellah, Zahra and Arrowsmith, Claire and Chillingworth, Tracey and Churcher, Carol and Clarke, Kay and Cronin, Ann and Davis, Paul and Goodhead, Ian and Holroyd, Nancy and Jagels, Kay and Lord, Angela and Moule, Sharon and Mungall, Karen and Norbertczak, Halina and Quail, Michael A. and Rabbinowitsch, Ester and Walker, Danielle and White, Brian and Whitehead, Sally and Small, Pamela L.C. and Brosch, Roland and Ramakrishnan, Lalita and Fischbach, Michael A. and Parkhill, Julian and Cole, Stewart T.}, 
title = {Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis},
volume = {18}, 
number = {5}, 
pages = {729-741}, 
year = {2008}, 
doi = {10.1101/gr.075069.107}, 
abstract ={Mycobacterium marinum, a ubiquitous pathogen of fish and amphibia, is a near relative of Mycobacterium tuberculosis, the etiologic agent of tuberculosis in humans. The genome of the M strain of M. marinum comprises a 6,636,827-bp circular chromosome with 5424 CDS, 10 prophages, and a 23-kb mercury-resistance plasmid. Prominent features are the very large number of genes (57) encoding polyketide synthases (PKSs) and nonribosomal peptide synthases (NRPSs) and the most extensive repertoire yet reported of the mycobacteria-restricted PE and PPE proteins, and related-ESX secretion systems. Some of the NRPS genes comprise a novel family and seem to have been acquired horizontally. M. marinum is used widely as a model organism to study M. tuberculosis pathogenesis, and genome comparisons confirmed the close genetic relationship between these two species, as they share 3000 orthologs with an average amino acid identity of 85%. Comparisons with the more distantly related Mycobacterium avium subspecies paratuberculosis and Mycobacterium smegmatis reveal how an ancestral generalist mycobacterium evolved into M. tuberculosis and M. marinum. M. tuberculosis has undergone genome downsizing and extensive lateral gene transfer to become a specialized pathogen of humans and other primates without retaining an environmental niche. M. marinum has maintained a large genome so as to retain the capacity for environmental survival while becoming a broad host range pathogen that produces disease strikingly similar to M. tuberculosis. The work described herein provides a foundation for using M. marinum to better understand the determinants of pathogenesis of tuberculosis.}, 
URL = {http://genome.cshlp.org/content/18/5/729.abstract}, 
eprint = {http://genome.cshlp.org/content/18/5/729.full.pdf+html}, 
journal = {Genome Research} 
}