TY  - JOUR
A1  - Reinders, Jon
A1  - Delucinge Vivier, Céline
A1  - Theiler, Grégory
A1  - Chollet, Didier
A1  - Descombes, Patrick
A1  - Paszkowski, Jerzy
T1  - Genome-wide, high-resolution DNA methylation profiling using bisulfite-mediated cytosine conversion
Y1  - 2008/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 469 
EP  - 476 
DO  - 10.1101/gr.7073008 
VL  - 18 
IS  - 3 
UR  - http://genome.cshlp.org/content/18/3/469.abstract 
N2  - Methylation of cytosines (mC) is essential for epigenetic gene regulation in plants and mammals. Aberrant mC patterns are associated with heritable developmental abnormalities in plants and with cancer in mammals. We have developed a genome-wide DNA methylation profiling technology employing a novel amplification step for DNA subjected to bisulfite-mediated cytosine conversion. The methylation patterns detected are not only consistent with previous results obtained with mC immunoprecipitation (mCIP) techniques, but also demonstrated improved resolution and sensitivity. The technology, named BiMP (for Bisulfite Methylation Profiling), is more cost-effective than mCIP and requires as little as 100 ng of Arabidopsis DNA. 
ER  -