RT Journal
A1 Hansen, Gwenn M.
A1 Markesich, Diane C.
A1 Burnett, Michael B.
A1 Zhu, Qichao
A1 Dionne, Karen M.
A1 Richter, Lizabeth J.
A1 Finnell, Richard H.
A1 Sands, Arthur T.
A1 Zambrowicz, Brian P.
A1 Abuin, Alejandro
T1 Large-scale gene trapping in C57BL/6N mouse embryonic stem cells
JF Genome Research 
JO Genome Research 
YR 2008 
FD October 01 
VO 18 
IS 10 
SP 1670 
OP 1679 
DO 10.1101/gr.078352.108 
UL http://genome.cshlp.org/content/18/10/1670.abstract 
AB We report the construction and analysis of a mouse gene trap mutant resource created in the C57BL/6N genetic background containing more than 350,000 sequence-tagged embryonic stem (ES) cell clones. We also demonstrate the ability of these ES cell clones to contribute to the germline and produce knockout mice. Each mutant clone is identified by a genomic sequence tag representing the exact insertion location, allowing accurate prediction of mutagenicity and enabling direct genotyping of mutant alleles. Mutations have been identified in more than 10,000 genes and show a bias toward the first intron. The trapped ES cell lines, which can be requested from the Texas A&M Institute for Genomic Medicine, are readily available to the scientific community.