RT Journal
A1 Garcia-Perez, José L.
A1 Doucet, Aurélien J.
A1 Bucheton, Alain
A1 Moran, John V.
A1 Gilbert, Nicolas
T1 Distinct mechanisms for trans-mediated mobilization of cellular RNAs by the LINE-1 reverse transcriptase
JF Genome Research 
JO Genome Research 
YR 2007 
FD May 01 
VO 17 
IS 5 
SP 602 
OP 611 
DO 10.1101/gr.5870107 
UL http://genome.cshlp.org/content/17/5/602.abstract 
AB Long Interspersed Element-1 (LINE-1 or L1) sequences comprise ∼17% of human DNA and ongoing L1 retrotransposition continues to impact genome evolution. The L1-encoded proteins also can mobilize other cellular RNAs (e.g., Alu retrotransposons, SVA retrotransposons, and U6 snRNAs), which comprise ∼13% of human DNA. Here, we demonstrate that the trans-mediated mobilization of non-L1 RNAs can occur by either template choice or template-switching mechanisms. Remarkably, these mechanisms are not mutually exclusive, as both processes can operate sequentially on the same RNA template. Finally, we provide evidence that efficient U6 snRNA retrotransposition requires both ORF1p and ORF2p, providing indirect evidence for the action of ORF1p in U6 snRNA retrotransposition. Thus, we propose that the LINE-1-encoded reverse transcriptase can mediate the retrotransposition of non-L1 RNAs by distinct mechanisms.