TY  - JOUR
A1  - Hu, Yanhui
A1  - Rolfs, Andreas
A1  - Bhullar, Bhupinder
A1  - Murthy, Tellamraju V. S.
A1  - Zhu, Cong
A1  - Berger, Michael F.
A1  - Camargo, Anamaria A.
A1  - Kelley, Fontina
A1  - McCarron, Seamus
A1  - Jepson, Daniel
A1  - Richardson, Aaron
A1  - Raphael, Jacob
A1  - Moreira, Donna
A1  - Taycher, Elena
A1  - Zuo, Dongmei
A1  - Mohr, Stephanie
A1  - Kane, Michael F.
A1  - Williamson, Janice
A1  - Simpson, Andrew
A1  - Bulyk, Martha L.
A1  - Harlow, Edward
A1  - Marsischky, Gerald
A1  - Kolodner, Richard D.
A1  - LaBaer, Joshua
T1  - Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae
Y1  - 2007/04/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 536 
EP  - 543 
DO  - 10.1101/gr.6037607 
VL  - 17 
IS  - 4 
UR  - http://genome.cshlp.org/content/17/4/536.abstract 
N2  - The availability of an annotated genome sequence for the yeast Saccharomyces cerevisiae has made possible the proteome-scale study of protein function and protein–protein interactions. These studies rely on availability of cloned open reading frame (ORF) collections that can be used for cell-free or cell-based protein expression. Several yeast ORF collections are available, but their use and data interpretation can be hindered by reliance on now out-of-date annotations, the inflexible presence of N- or C-terminal tags, and/or the unknown presence of mutations introduced during the cloning process. High-throughput biochemical and genetic analyses would benefit from a “gold standard” (fully sequence-verified, high-quality) ORF collection, which allows for high confidence in and reproducibility of experimental results. Here, we describe Yeast FLEXGene, a S. cerevisiae protein-coding clone collection that covers over 5000 predicted protein-coding sequences. The clone set covers 87% of the current S. cerevisiae genome annotation and includes full sequencing of each ORF insert. Availability of this collection makes possible a wide variety of studies from purified proteins to mutation suppression analysis, which should contribute to a global understanding of yeast protein function. 
ER  -