TY  - JOUR
A1  - Jehan, Zeenath
A1  - Vallinayagam, Sambandam
A1  - Tiwari, Shrish
A1  - Pradhan, Suman
A1  - Singh, Lalji
A1  - Suresh, Amritha
A1  - Reddy, Hemakumar M.
A1  - Ahuja, Y.R.
A1  - Jesudasan, Rachel A.
T1  - Novel noncoding RNA from human Y distal heterochromatic block (Yq12) generates testis-specific chimeric CDC2L2
Y1  - 2007/04/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 433 
EP  - 440 
DO  - 10.1101/gr.5155706 
VL  - 17 
IS  - 4 
UR  - http://genome.cshlp.org/content/17/4/433.abstract 
N2  - The human Y chromosome, because it is enriched in repetitive DNA, has been very intractable to genetic and molecular analyses. There is no previous evidence for developmental stage- and testis-specific transcription from the male-specific region of the Y (MSY). Here, we present evidence for the first time for a developmental stage- and testis-specific transcription from MSY distal heterochromatic block. We isolated two novel RNAs, which localize to Yq12 in multiple copies, show testis-specific expression, and lack active X-homologs. Experimental evidence shows that one of the above Yq12 noncoding RNAs (ncRNAs) trans-splices with CDC2L2 mRNA from chromosome 1p36.3 locus to generate a testis-specific chimeric β sv13 isoform. This 67-nt 5′UTR provided by the Yq12 transcript contains within it a Y box protein-binding CCAAT motif, indicating translational regulation of the β sv13 isoform in testis. This is also the first report of trans-splicing between a Y chromosomal and an autosomal transcript. 
ER  -