RT Journal
A1 Maydan, Jason S.
A1 Flibotte, Stephane
A1 Edgley, Mark L.
A1 Lau, Joanne
A1 Selzer, Rebecca R.
A1 Richmond, Todd A.
A1 Pofahl, Nathan J.
A1 Thomas, James H.
A1 Moerman, Donald G.
T1 Efficient high-resolution deletion discovery in Caenorhabditis elegans by array comparative genomic hybridization
JF Genome Research 
JO Genome Research 
YR 2007 
FD March 01 
VO 17 
IS 3 
SP 337 
OP 347 
DO 10.1101/gr.5690307 
UL http://genome.cshlp.org/content/17/3/337.abstract 
AB We have developed array Comparative Genomic Hybridization for Caenorhabditis elegans as a means of screening for novel induced deletions in this organism. We designed three microarrays consisting of overlapping 50-mer probes to annotated exons and micro-RNAs, the first with probes to chromosomes X and II, the second with probes to chromosome II alone, and a third to the entire genome. These arrays were used to reliably detect both a large (50 kb) multigene deletion and a small (1 kb) single-gene deletion in homozygous and heterozygous samples. In one case, a deletion breakpoint was resolved to fewer than 50 bp. In an experiment designed to identify new mutations we used the X:II and II arrays to detect deletions associated with lethal mutants on chromosome II. One is an 8-kb deletion targeting the ast-1 gene on chromosome II and another is a 141-bp deletion in the gene C06A8.1. Others span large sections of the chromosome, up to >750 kb. As a further application of array Comparative Genomic Hybridization in C. elegans we used the whole-genome array to detect the extensive natural gene content variation (almost 2%) between the N2 Bristol strain and the strain CB4856, a strain isolated in Hawaii and JU258, a strain isolated in Madeira.