TY  - JOUR
A1  - Maydan, Jason S.
A1  - Flibotte, Stephane
A1  - Edgley, Mark L.
A1  - Lau, Joanne
A1  - Selzer, Rebecca R.
A1  - Richmond, Todd A.
A1  - Pofahl, Nathan J.
A1  - Thomas, James H.
A1  - Moerman, Donald G.
T1  - Efficient high-resolution deletion discovery in Caenorhabditis elegans by array comparative genomic hybridization
Y1  - 2007/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 337 
EP  - 347 
DO  - 10.1101/gr.5690307 
VL  - 17 
IS  - 3 
UR  - http://genome.cshlp.org/content/17/3/337.abstract 
N2  - We have developed array Comparative Genomic Hybridization for Caenorhabditis elegans as a means of screening for novel induced deletions in this organism. We designed three microarrays consisting of overlapping 50-mer probes to annotated exons and micro-RNAs, the first with probes to chromosomes X and II, the second with probes to chromosome II alone, and a third to the entire genome. These arrays were used to reliably detect both a large (50 kb) multigene deletion and a small (1 kb) single-gene deletion in homozygous and heterozygous samples. In one case, a deletion breakpoint was resolved to fewer than 50 bp. In an experiment designed to identify new mutations we used the X:II and II arrays to detect deletions associated with lethal mutants on chromosome II. One is an 8-kb deletion targeting the ast-1 gene on chromosome II and another is a 141-bp deletion in the gene C06A8.1. Others span large sections of the chromosome, up to >750 kb. As a further application of array Comparative Genomic Hybridization in C. elegans we used the whole-genome array to detect the extensive natural gene content variation (almost 2%) between the N2 Bristol strain and the strain CB4856, a strain isolated in Hawaii and JU258, a strain isolated in Madeira. 
ER  -