RT Journal
A1 Peters, Brock A.
A1 St. Croix, Brad
A1 Sjöblom, Tobias
A1 Cummins, Jordan M.
A1 Silliman, Natalie
A1 Ptak, Janine
A1 Saha, Saurabh
A1 Kinzler, Kenneth W.
A1 Hatzis, Christos
A1 Velculescu, Victor E.
T1 Large-scale identification of novel transcripts in the human genome
JF Genome Research 
JO Genome Research 
YR 2007 
FD March 01 
VO 17 
IS 3 
SP 287 
OP 292 
DO 10.1101/gr.5486607 
UL http://genome.cshlp.org/content/17/3/287.abstract 
AB Although the sequencing of the human genome has been completed, the number and identity of genes contained within it remains to be fully determined. We used LongSAGE to analyze 660,357 human transcripts from human brain mRNA and identified expression of 17,409 known genes and >15,000 different transcripts that were not annotated in genome databases. Analysis of a subset of these unannotated transcripts suggests that 85% were differentially expressed in various tissue types and that fewer than 20% would have been detected by ab initio gene predictions. These studies suggest that the human genome contains on the order of twice as many transcribed regions as are currently annotated and that experimental approaches will be required to fully elucidate the novel genes corresponding to these transcripts.