RT Journal
A1 Pontius, Joan U.
A1 Mullikin, James C.
A1 Smith, Douglas R.
A1 Agencourt Sequencing Team
A1 Lindblad-Toh, Kerstin
A1 Gnerre, Sante
A1 Clamp, Michele
A1 Chang, Jean
A1 Stephens, Robert
A1 Neelam, Beena
A1 Volfovsky, Natalia
A1 Schäffer, Alejandro A.
A1 Agarwala, Richa
A1 Narfström, Kristina
A1 Murphy, William J.
A1 Giger, Urs
A1 Roca, Alfred L.
A1 Antunes, Agostinho
A1 Menotti-Raymond, Marilyn
A1 Yuhki, Naoya
A1 Pecon-Slattery, Jill
A1 Johnson, Warren E.
A1 Bourque, Guillaume
A1 Tesler, Glenn
A1 NISC Comparative Sequencing Program
A1 O’Brien, Stephen J.
T1 Initial sequence and comparative analysis of the cat genome
JF Genome Research 
JO Genome Research 
YR 2007 
FD November 01 
VO 17 
IS 11 
SP 1675 
OP 1689 
DO 10.1101/gr.6380007 
UL http://genome.cshlp.org/content/17/11/1675.abstract 
AB The genome sequence (1.9-fold coverage) of an inbred Abyssinian domestic cat was assembled, mapped, and annotated with a comparative approach that involved cross-reference to annotated genome assemblies of six mammals (human, chimpanzee, mouse, rat, dog, and cow). The results resolved chromosomal positions for 663,480 contigs, 20,285 putative feline gene orthologs, and 133,499 conserved sequence blocks (CSBs). Additional annotated features include repetitive elements, endogenous retroviral sequences, nuclear mitochondrial (numt) sequences, micro-RNAs, and evolutionary breakpoints that suggest historic balancing of translocation and inversion incidences in distinct mammalian lineages. Large numbers of single nucleotide polymorphisms (SNPs), deletion insertion polymorphisms (DIPs), and short tandem repeats (STRs), suitable for linkage or association studies were characterized in the context of long stretches of chromosome homozygosity. In spite of the light coverage capturing ∼65% of euchromatin sequence from the cat genome, these comparative insights shed new light on the tempo and mode of gene/genome evolution in mammals, promise several research applications for the cat, and also illustrate that a comparative approach using more deeply covered mammals provides an informative, preliminary annotation of a light (1.9-fold) coverage mammal genome sequence.