TY  - JOUR
A1  - Bibikova, Marina
A1  - Chudin, Eugene
A1  - Wu, Bonnie
A1  - Zhou, Lixin
A1  - Garcia, Eliza Wickham
A1  - Liu, Ying
A1  - Shin, Soojung
A1  - Plaia, Todd W.
A1  - Auerbach, Jonathan M.
A1  - Arking, Dan E.
A1  - Gonzalez, Rodolfo
A1  - Crook, Jeremy
A1  - Davidson, Bruce
A1  - Schulz, Thomas C.
A1  - Robins, Allan
A1  - Khanna, Aparna
A1  - Sartipy, Peter
A1  - Hyllner, Johan
A1  - Vanguri, Padmavathy
A1  - Savant-Bhonsale, Smita
A1  - Smith, Alan K.
A1  - Chakravarti, Aravinda
A1  - Maitra, Anirban
A1  - Rao, Mahendra
A1  - Barker, David L.
A1  - Loring, Jeanne F.
A1  - Fan, Jian-Bing
T1  - Human embryonic stem cells have a unique epigenetic signature
Y1  - 2006/09/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1075 
EP  - 1083 
DO  - 10.1101/gr.5319906 
VL  - 16 
IS  - 9 
UR  - http://genome.cshlp.org/content/16/9/1075.abstract 
N2  - Human embryonic stem (hES) cells originate during an embryonic period of active epigenetic remodeling. DNA methylation patterns are likely to be critical for their self-renewal and pluripotence. We compared the DNA methylation status of 1536 CpG sites (from 371 genes) in 14 independently isolated hES cell lines with five other cell types: 24 cancer cell lines, four adult stem cell populations, four lymphoblastoid cell lines, five normal human tissues, and an embryonal carcinoma cell line. We found that the DNA methylation profile clearly distinguished the hES cells from all of the other cell types. A subset of 49 CpG sites from 40 genes contributed most to the differences among cell types. Another set of 25 sites from 23 genes distinguished hES cells from normal differentiated cells and can be used as biomarkers to monitor differentiation. Our results indicate that hES cells have a unique epigenetic signature that may contribute to their developmental potential. 
ER  -