@article{Morin01062006,
author = {Morin, Ryan D. and Chang, Elbert and Petrescu, Anca and Liao, Nancy and Griffith, Malachi and Kirkpatrick, Robert and Butterfield, Yaron S. and Young, Alice C. and Stott, Jeffrey and Barber, Sarah and Babakaiff, Ryan and Dickson, Mark C. and Matsuo, Corey and Wong, David and Yang, George S. and Smailus, Duane E. and Wetherby, Keith D. and Kwong, Peggy N. and Grimwood, Jane and Brinkley, Charles P. and Brown-John, Mabel and Reddix-Dugue, Natalie D. and Mayo, Michael and Schmutz, Jeremy and Beland, Jaclyn and Park, Morgan and Gibson, Susan and Olson, Teika and Bouffard, Gerard G. and Tsai, Miranda and Featherstone, Ruth and Chand, Steve and Siddiqui, Asim S. and Jang, Wonhee and Lee, Ed and Klein, Steven L. and Blakesley, Robert W. and Zeeberg, Barry R. and Narasimhan, Sudarshan and Weinstein, John N. and Pennacchio, Christa Prange and Myers, Richard M. and Green, Eric D. and Wagner, Lukas and Gerhard, Daniela S. and Marra, Marco A. and Jones, Steven J.M. and Holt, Robert A.}, 
title = {Sequencing and analysis of 10,967 full-length cDNA clones from Xenopus laevis and Xenopus tropicalis reveals post-tetraploidization transcriptome remodeling},
volume = {16}, 
number = {6}, 
pages = {796-803}, 
year = {2006}, 
doi = {10.1101/gr.4871006}, 
abstract ={Sequencing of full-insert clones from full-length cDNA libraries from both Xenopus laevis and Xenopus tropicalis has been ongoing as part of the Xenopus Gene Collection Initiative. Here we present 10,967 full ORF verified cDNA clones (8049 from X. laevis and 2918 from X. tropicalis) as a community resource. Because the genome of X. laevis, but not X. tropicalis, has undergone allotetraploidization, comparison of coding sequences from these two clawed (pipid) frogs provides a unique angle for exploring the molecular evolution of duplicate genes. Within our clone set, we have identified 445 gene trios, each comprised of an allotetraploidization-derived X. laevis gene pair and their shared X. tropicalis ortholog. Pairwise dN/dS, comparisons within trios show strong evidence for purifying selection acting on all three members. However, dN/dS ratios between X. laevis gene pairs are elevated relative to their X. tropicalis ortholog. This difference is highly significant and indicates an overall relaxation of selective pressures on duplicated gene pairs. We have found that the paralogs that have been lost since the tetraploidization event are enriched for several molecular functions, but have found no such enrichment in the extant paralogs. Approximately 14% of the paralogous pairs analyzed here also show differential expression indicative of subfunctionalization.}, 
URL = {http://genome.cshlp.org/content/16/6/796.abstract}, 
eprint = {http://genome.cshlp.org/content/16/6/796.full.pdf+html}, 
journal = {Genome Research} 
}