RT Journal
A1 Arifuzzaman, Mohammad
A1 Maeda, Maki
A1 Itoh, Aya
A1 Nishikata, Kensaku
A1 Takita, Chiharu
A1 Saito, Rintaro
A1 Ara, Takeshi
A1 Nakahigashi, Kenji
A1 Huang, Hsuan-Cheng
A1 Hirai, Aki
A1 Tsuzuki, Kohei
A1 Nakamura, Seira
A1 Altaf-Ul-Amin, Mohammad
A1 Oshima, Taku
A1 Baba, Tomoya
A1 Yamamoto, Natsuko
A1 Kawamura, Tomoyo
A1 Ioka-Nakamichi, Tomoko
A1 Kitagawa, Masanari
A1 Tomita, Masaru
A1 Kanaya, Shigehiko
A1 Wada, Chieko
A1 Mori, Hirotada
T1 Large-scale identification of protein–protein interaction of Escherichia coli K-12
JF Genome Research 
JO Genome Research 
YR 2006 
FD May 01 
VO 16 
IS 5 
SP 686 
OP 691 
DO 10.1101/gr.4527806 
UL http://genome.cshlp.org/content/16/5/686.abstract 
AB Protein–protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested, partners were found for 2667, including 779 of unknown function. Proteins copurifying with hexahistidine-tagged baits on a Ni2+-NTA column were identified by MALDI-TOF MS (matrix-assisted laser desorption ionization time of flight mass spectrometry). An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology.