TY  - JOUR
A1  - Arifuzzaman, Mohammad
A1  - Maeda, Maki
A1  - Itoh, Aya
A1  - Nishikata, Kensaku
A1  - Takita, Chiharu
A1  - Saito, Rintaro
A1  - Ara, Takeshi
A1  - Nakahigashi, Kenji
A1  - Huang, Hsuan-Cheng
A1  - Hirai, Aki
A1  - Tsuzuki, Kohei
A1  - Nakamura, Seira
A1  - Altaf-Ul-Amin, Mohammad
A1  - Oshima, Taku
A1  - Baba, Tomoya
A1  - Yamamoto, Natsuko
A1  - Kawamura, Tomoyo
A1  - Ioka-Nakamichi, Tomoko
A1  - Kitagawa, Masanari
A1  - Tomita, Masaru
A1  - Kanaya, Shigehiko
A1  - Wada, Chieko
A1  - Mori, Hirotada
T1  - Large-scale identification of protein–protein interaction of Escherichia coli K-12
Y1  - 2006/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 686 
EP  - 691 
DO  - 10.1101/gr.4527806 
VL  - 16 
IS  - 5 
UR  - http://genome.cshlp.org/content/16/5/686.abstract 
N2  - Protein–protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested, partners were found for 2667, including 779 of unknown function. Proteins copurifying with hexahistidine-tagged baits on a Ni2+-NTA column were identified by MALDI-TOF MS (matrix-assisted laser desorption ionization time of flight mass spectrometry). An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology. 
ER  -