RT Journal
A1 Shoguchi, Eiichi
A1 Kawashima, Takeshi
A1 Satou, Yutaka
A1 Hamaguchi, Makoto
A1 Sin-I, Tadasu
A1 Kohara, Yuji
A1 Putnam, Nik
A1 Rokhsar, Daniel S.
A1 Satoh, Nori
T1 Chromosomal mapping of 170 BAC clones in the ascidian Ciona intestinalis
JF Genome Research 
JO Genome Research 
YR 2006 
FD February 01 
VO 16 
IS 2 
SP 297 
OP 303 
DO 10.1101/gr.4156606 
UL http://genome.cshlp.org/content/16/2/297.abstract 
AB The draft genome (∼160 Mb) of the urochordate ascidian Ciona intestinalis has been sequenced by the whole-genome shotgun method and should provide important insights into the origin and evolution of chordates as well as vertebrates. However, because this genomic data has not yet been mapped onto chromosomes, important biological questions including regulation of gene expression at the genome-wide level cannot yet be addressed. Here, we report the molecular cytogenetic characterization of all 14 pairs of C. intestinalis chromosomes, as well as initial large-scale mapping of genomic sequences onto chromosomes by fluorescent in situ hybridization (FISH). Two-color FISH using 170 bacterial artificial chromosome (BAC) clones and construction of joined scaffolds using paired BAC end sequences allowed for mapping of up to 65% of the deduced 117-Mb nonrepetitive sequence onto chromosomes. This map lays the foundation for future studies of the protochordate C. intestinalis genome at the chromosomal level.