@article{Babushok01022006,
author = {Babushok, Daria V. and Ostertag, Eric M. and Courtney, Christine E. and Choi, Janice M. and Kazazian, Haig H.}, 
title = {L1 integration in a transgenic mouse model},
volume = {16}, 
number = {2}, 
pages = {240-250}, 
year = {2006}, 
doi = {10.1101/gr.4571606}, 
abstract ={To study integration of the human LINE-1 retrotransposon (L1) in vivo, we developed a transgenic mouse model of L1 retrotransposition that displays de novo somatic L1 insertions at a high frequency, occasionally several insertions per mouse. We mapped 3′ integration sites of 51 insertions by Thermal Asymmetric Interlaced PCR (TAIL–PCR). Analysis of integration locations revealed a broad genomic distribution with a modest preference for intergenic regions. We characterized the complete structures of 33 de novo retrotransposition events. Our results highlight the large number of highly truncated L1s, as over 52% (27/51) of total integrants were <1/3 the length of a full-length element. New integrants carry all structural characteristics typical of genomic L1s, including a number with inversions, deletions, and 5′-end microhomologies to the target DNA sequence. Notably, at least 13% (7/51) of all insertions contain a short stretch of extra nucleotides at their 5′ end, which we postulate result from template-jumping by the L1-encoded reverse transcriptase. We propose a unified model of L1 integration that explains all of the characteristic features of L1 retrotransposition, such as 5′ truncations, inversions, extra nucleotide additions, and 5′ boundary and inversion point microhomologies.}, 
URL = {http://genome.cshlp.org/content/16/2/240.abstract}, 
eprint = {http://genome.cshlp.org/content/16/2/240.full.pdf+html}, 
journal = {Genome Research} 
}