RT Journal
A1 Elnitski, Laura
A1 Jin, Victor X.
A1 Farnham, Peggy J.
A1 Jones, Steven J.M.
T1 Locating mammalian transcription factor binding sites: A survey of computational and experimental techniques
JF Genome Research 
JO Genome Research 
YR 2006 
FD December 01 
VO 16 
IS 12 
SP 1455 
OP 1464 
DO 10.1101/gr.4140006 
UL http://genome.cshlp.org/content/16/12/1455.abstract 
AB Fields such as genomics and systems biology are built on the synergism between computational and experimental techniques. This type of synergism is especially important in accomplishing goals like identifying all functional transcription factor binding sites in vertebrate genomes. Precise detection of these elements is a prerequisite to deciphering the complex regulatory networks that direct tissue specific and lineage specific patterns of gene expression. This review summarizes approaches for in silico, in vitro, and in vivo identification of transcription factor binding sites. A variety of techniques useful for localized- and high-throughput analyses are discussed here, with emphasis on aspects of data generation and verification.