RT Journal
A1 Berezikov, Eugene
A1 van Tetering, Geert
A1 Verheul, Mark
A1 van de Belt, Jose
A1 van Laake, Linda
A1 Vos, Joost
A1 Verloop, Robert
A1 van de Wetering, Marc
A1 Guryev, Victor
A1 Takada, Shuji
A1 van Zonneveld, Anton Jan
A1 Mano, Hiroyuki
A1 Plasterk, Ronald
A1 Cuppen, Edwin
T1 Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis
JF Genome Research 
JO Genome Research 
YR 2006 
FD October 01 
VO 16 
IS 10 
SP 1289 
OP 1298 
DO 10.1101/gr.5159906 
UL http://genome.cshlp.org/content/16/10/1289.abstract 
AB icroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently >400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ~10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted.