TY  - JOUR
A1  - Berezikov, Eugene
A1  - van Tetering, Geert
A1  - Verheul, Mark
A1  - van de Belt, Jose
A1  - van Laake, Linda
A1  - Vos, Joost
A1  - Verloop, Robert
A1  - van de Wetering, Marc
A1  - Guryev, Victor
A1  - Takada, Shuji
A1  - van Zonneveld, Anton Jan
A1  - Mano, Hiroyuki
A1  - Plasterk, Ronald
A1  - Cuppen, Edwin
T1  - Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis
Y1  - 2006/10/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1289 
EP  - 1298 
DO  - 10.1101/gr.5159906 
VL  - 16 
IS  - 10 
UR  - http://genome.cshlp.org/content/16/10/1289.abstract 
N2  - icroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently >400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ~10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted. 
ER  -