@article{Berezikov01102006,
author = {Berezikov, Eugene and van Tetering, Geert and Verheul, Mark and van de Belt, Jose and van Laake, Linda and Vos, Joost and Verloop, Robert and van de Wetering, Marc and Guryev, Victor and Takada, Shuji and van Zonneveld, Anton Jan and Mano, Hiroyuki and Plasterk, Ronald and Cuppen, Edwin}, 
title = {Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis},
volume = {16}, 
number = {10}, 
pages = {1289-1298}, 
year = {2006}, 
doi = {10.1101/gr.5159906}, 
abstract ={icroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently >400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ~10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted.}, 
URL = {http://genome.cshlp.org/content/16/10/1289.abstract}, 
eprint = {http://genome.cshlp.org/content/16/10/1289.full.pdf+html}, 
journal = {Genome Research} 
}