TY  - JOUR
A1  - Drouaud, Jan
A1  - Camilleri, Christine
A1  - Bourguignon, Pierre-Yves
A1  - Canaguier, Aurélie
A1  - Bérard, Aurélie
A1  - Vezon, Daniel
A1  - Giancola, Sandra
A1  - Brunel, Dominique
A1  - Colot, Vincent
A1  - Prum, Bernard
A1  - Quesneville, Hadi
A1  - Mézard, Christine
T1  - Variation in crossing-over rates across chromosome 4 of Arabidopsis thaliana reveals the presence of meiotic recombination “hot spots”
Y1  - 2006/01/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 106 
EP  - 114 
DO  - 10.1101/gr.4319006 
VL  - 16 
IS  - 1 
UR  - http://genome.cshlp.org/content/16/1/106.abstract 
N2  - Crossover (CO) is a key process for the accurate segregation of homologous chromosomes during the first meiotic division. In most eukaryotes, meiotic recombination is not homogeneous along the chromosomes, suggesting a tight control of the location of recombination events. We genotyped 71 single nucleotide polymorphisms (SNPs) covering the entire chromosome 4 of Arabidopsis thaliana on 702 F2 plants, representing 1404 meioses and allowing the detection of 1171 COs, to study CO localization in a higher plant. The genetic recombination rates varied along the chromosome from 0 cM/Mb near the centromere to 20 cM/Mb on the short arm next to the NOR region, with a chromosome average of 4.6 cM/Mb. Principal component analysis showed that CO rates negatively correlate with the G+C content (P =3×10-4), in contrast to that reported in other eukaryotes. COs also significantly correlate with the density of single repeats and the CpG ratio, but not with genes, pseudogenes, transposable elements, or dispersed repeats. Chromosome 4 has, on average, 1.6 COs per meiosis, and these COs are subjected to interference. A detailed analysis of several regions having high CO rates revealed “hot spots” of meiotic recombination contained in small fragments of a few kilobases. Both the intensity and the density of these hot spots explain the variation of CO rates along the chromosome. 
ER  -