RT Journal
A1 González, Josefa
A1 Nefedov, Michael
A1 Bosdet, Ian
A1 Casals, Ferran
A1 Calvete, Oriol
A1 Delprat, Alejandra
A1 Shin, Heesun
A1 Chiu, Readman
A1 Mathewson, Carrie
A1 Wye, Natasja
A1 Hoskins, Roger A.
A1 Schein, Jacqueline E.
A1 de Jong, Pieter
A1 Ruiz, Alfredo
T1 A BAC-based physical map of the Drosophila buzzatii genome
JF Genome Research 
JO Genome Research 
YR 2005 
FD June 01 
VO 15 
IS 6 
SP 885 
OP 892 
DO 10.1101/gr.3263105 
UL http://genome.cshlp.org/content/15/6/885.abstract 
AB Large-insert genomic libraries facilitate cloning of large genomic regions, allow the construction of clone-based physical maps, and provide useful resources for sequencing entire genomes. Drosophila buzzatii is a representative species of the repleta group in the Drosophila subgenus, which is being widely used as a model in studies of genome evolution, ecological adaptation, and speciation. We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vector pTARBAC2.1. The library comprises 18,353 clones with an average insert size of 152 kb and an ∼18× expected representation of the D. buzzatii euchromatic genome. We screened the entire library with six euchromatic gene probes and estimated the actual genome representation to be ∼23×. In addition, we fingerprinted by restriction digestion and agarose gel electrophoresis a sample of 9555 clones, and assembled them using FingerPrint Contigs (FPC) software and manual editing into 345 contigs (mean of 26 clones per contig) and 670 singletons. Finally, we anchored 181 large contigs (containing 7788 clones) to the D. buzzatii salivary gland polytene chromosomes by in situ hybridization of 427 representative clones. The BAC library and a database with all the information regarding the high coverage BAC-based physical map described in this paper are available to the research community.