TY  - JOUR
A1  - Liu, Xiao
A1  - Noll, David M.
A1  - Lieb, Jason D.
A1  - Clarke, Neil D.
T1  - DIP-chip: Rapid and accurate determination of DNA-binding specificity
Y1  - 2005/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 421 
EP  - 427 
DO  - 10.1101/gr.3256505 
VL  - 15 
IS  - 3 
UR  - http://genome.cshlp.org/content/15/3/421.abstract 
N2  - We have developed a new method for determining the DNA-binding specificity of proteins. In DIP-chip (DNA immunoprecipitation with microarray detection), protein·DNA complexes are isolated from an in vitro mixture of purified protein and naked genomic DNA. Whole-genome DNA microarrays are used to identify the protein-bound DNA fragments, and the sequence of the identified fragments is used to derive binding-site descriptions. Using objective criteria for assessing the accuracy of DNA-binding motifs, and using yeast Leu3p as a model, we demonstrate that motifs determined by DIP-chip are as effective at predicting the location of bound proteins in vivo as are motifs determined by conventional low-throughput in vitro methods. 
ER  -