RT Journal
A1 Milosavljevic, Aleksandar
A1 Harris, Ronald A.
A1 Sodergren, Erica J.
A1 Jackson, Andrew R.
A1 Kalafus, Ken J.
A1 Hodgson, Anne
A1 Cree, Andrew
A1 Dai, Weilie
A1 Csuros, Miklos
A1 Zhu, Baoli
A1 de Jong, Pieter J.
A1 Weinstock, George M.
A1 Gibbs, Richard A.
T1 Pooled genomic indexing of rhesus macaque
JF Genome Research 
JO Genome Research 
YR 2005 
FD February 01 
VO 15 
IS 2 
SP 292 
OP 301 
DO 10.1101/gr.3162505 
UL http://genome.cshlp.org/content/15/2/292.abstract 
AB Pooled genomic indexing (PGI) is a method for mapping collections of bacterial artificial chromosome (BAC) clones between species by using a combination of clone pooling and DNA sequencing. PGI has been used to map a total of 3858 BAC clones covering ∼24% of the rhesus macaque (Macaca mulatta) genome onto 4178 homologous loci in the human genome. A number of intrachromosomal rearrangements were detected by mapping multiple segments within the individual rhesus BACs onto multiple disjoined loci in the human genome. Transversal pooling designs involving shuffled BAC arrays were employed for robust mapping even with modest DNA sequence read coverage. A further innovation, short-tag pooled genomic indexing (ST-PGI), was also introduced to further improve the economy of mapping by sequencing multiple, short, mapable tags within a single sequencing reaction.