RT Journal
A1 Metzker, Michael L.
T1 Emerging technologies in DNA sequencing
JF Genome Research 
JO Genome Research 
YR 2005 
FD December 01 
VO 15 
IS 12 
SP 1767 
OP 1776 
DO 10.1101/gr.3770505 
UL http://genome.cshlp.org/content/15/12/1767.abstract 
AB Demand for DNA sequence information has never been greater, yet current Sanger technology is too costly, time consuming, and labor intensive to meet this ongoing demand. Applications span numerous research interests, including sequence variation studies, comparative genomics and evolution, forensics, and diagnostic and applied therapeutics. Several emerging technologies show promise of delivering next-generation solutions for fast and affordable genome sequencing. In this review article, the DNA polymerase-dependent strategies of Sanger sequencing, single nucleotide addition, and cyclic reversible termination are discussed to highlight recent advances and potential challenges these technologies face in their development for ultrafast DNA sequencing.