TY  - JOUR
A1  - Smailus, Duane E.
A1  - Marziali, Andre
A1  - Dextras, Philip
A1  - Marra, Marco A.
A1  - Holt, Robert A.
T1  - Simple, robust methods for high-throughput nanoliter-scale DNA sequencing
Y1  - 2005/10/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1447 
EP  - 1450 
DO  - 10.1101/gr.4221805 
VL  - 15 
IS  - 10 
UR  - http://genome.cshlp.org/content/15/10/1447.abstract 
N2  - We have developed high-throughput DNA sequencing methods that generate high quality data from reactions as small as 400 nL, providing an approximate order of magnitude reduction in reagent use relative to standard protocols. Sequencing of clones from plasmid, fosmid, and BAC libraries yielded read lengths (PHRED20 bases) of 765 ± 172 (n = 10,272), 621 ± 201 (n = 1824), and 647 ± 189 (n = 568), respectively. Implementation of these procedures at high-throughput genome centers could have a substantial impact on the amount of data that can be generated per unit cost. 
ER  -