RT Journal
A1 Jaffe, Jacob D.
A1 Stange-Thomann, Nicole
A1 Smith, Cherylyn
A1 DeCaprio, David
A1 Fisher, Sheila
A1 Butler, Jonathan
A1 Calvo, Sarah
A1 Elkins, Tim
A1 FitzGerald, Michael G.
A1 Hafez, Nabil
A1 Kodira, Chinnappa D.
A1 Major, John
A1 Wang, Shunguang
A1 Wilkinson, Jane
A1 Nicol, Robert
A1 Nusbaum, Chad
A1 Birren, Bruce
A1 Berg, Howard C.
A1 Church, George M.
T1 The Complete Genome and Proteome of Mycoplasma mobile
JF Genome Research 
JO Genome Research 
YR 2004 
FD August 01 
VO 14 
IS 8 
SP 1447 
OP 1461 
DO 10.1101/gr.2674004 
UL http://genome.cshlp.org/content/14/8/1447.abstract 
AB Although often considered “minimal” organisms, mycoplasmas show a wide range of diversity with respect to host environment, phenotypic traits, and pathogenicity. Here we report the complete genomic sequence and proteogenomic map for the piscine mycoplasma Mycoplasma mobile, noted for its robust gliding motility. For the first time, proteomic data are used in the primary annotation of a new genome, providing validation of expression for many of the predicted proteins. Several novel features were discovered including a long repeating unit of DNA of ∼2435 bp present in five complete copies that are shown to code for nearly identical yet uniquely expressed proteins. M. mobile has among the lowest DNA GC contents (24.9%) and most reduced set of tRNAs of any organism yet reported (28). Numerous instances of tandem duplication as well as lateral gene transfer are evident in the genome. The multiple available complete genome sequences for other motile and immotile mycoplasmas enabled us to use comparative genomic and phylogenetic methods to suggest several candidate genes that might be involved in motility. The results of these analyses leave open the possibility that gliding motility might have arisen independently more than once in the mycoplasma lineage.