TY  - JOUR
A1  - Colland, Frédéric
A1  - Jacq, Xavier
A1  - Trouplin, Virginie
A1  - Mougin, Christelle
A1  - Groizeleau, Caroline
A1  - Hamburger, Alexandre
A1  - Meil, Alain
A1  - Wojcik, Jérôme
A1  - Legrain, Pierre
A1  - Gauthier, Jean-Michel
T1  - Functional Proteomics Mapping of a Human Signaling Pathway
Y1  - 2004/07/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1324 
EP  - 1332 
DO  - 10.1101/gr.2334104 
VL  - 14 
IS  - 7 
UR  - http://genome.cshlp.org/content/14/7/1324.abstract 
N2  - Access to the human genome facilitates extensive functional proteomics studies. Here, we present an integrated approach combining large-scale protein interaction mapping, exploration of the interaction network, and cellular functional assays performed on newly identified proteins involved in a human signaling pathway. As a proof of principle, we studied the Smad signaling system, which is regulated by members of the transforming growth factor β (TGFβ) superfamily. We used two-hybrid screening to map Smad signaling protein–protein interactions and to establish a network of 755 interactions, involving 591 proteins, 179 of which were poorly or not annotated. The exploration of such complex interaction databases is improved by the use of PIMRider, a dedicated navigation tool accessible through the Web. The biological meaning of this network is illustrated by the presence of 18 known Smad-associated proteins. Functional assays performed in mammalian cells including siRNA knock-down experiments identified eight novel proteins involved in Smad signaling, thus validating this integrated functional proteomics approach. 
ER  -