RT Journal
A1 Barker, David L.
A1 Hansen, Mark S.T.
A1 Faruqi, A. Fawad
A1 Giannola, Diane
A1 Irsula, Orlando R.
A1 Lasken, Roger S.
A1 Latterich, Martin
A1 Makarov, Vladimir
A1 Oliphant, Arnold
A1 Pinter, Jonathon H.
A1 Shen, Richard
A1 Sleptsova, Irina
A1 Ziehler, William
A1 Lai, Eric
T1 Two Methods of Whole-Genome Amplification Enable Accurate Genotyping Across a 2320-SNP Linkage Panel
JF Genome Research 
JO Genome Research 
YR 2004 
FD May 01 
VO 14 
IS 5 
SP 901 
OP 907 
DO 10.1101/gr.1949704 
UL http://genome.cshlp.org/content/14/5/901.abstract 
AB Comprehensive genome scans involving many thousands of SNP assays will require significant amounts of genomic DNA from each sample. We report two successful methods for amplifying whole-genomic DNA prior to SNP analysis, multiple displacement amplification, and OmniPlex technology. We determined the coverage of amplification by analyzing a SNP linkage marker set that contained 2320 SNP markers spread across the genome at an average distance of 2.5 cM. We observed a concordance of >99.8% in genotyping results from genomic DNA and amplified DNA, strongly indicating the ability of both methods used to amplify genomic DNA in a highly representative manner. Furthermore, we were able to achieve a SNP call rate of >98% in both genomic and amplified DNA. The combination of whole-genome amplification and comprehensive SNP linkage analysis offers new opportunities for genetic analysis in clinical trials, disease association studies, and archiving of DNA samples.