TY  - JOUR
A1  - Barker, David L.
A1  - Hansen, Mark S.T.
A1  - Faruqi, A. Fawad
A1  - Giannola, Diane
A1  - Irsula, Orlando R.
A1  - Lasken, Roger S.
A1  - Latterich, Martin
A1  - Makarov, Vladimir
A1  - Oliphant, Arnold
A1  - Pinter, Jonathon H.
A1  - Shen, Richard
A1  - Sleptsova, Irina
A1  - Ziehler, William
A1  - Lai, Eric
T1  - Two Methods of Whole-Genome Amplification Enable Accurate Genotyping Across a 2320-SNP Linkage Panel
Y1  - 2004/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 901 
EP  - 907 
DO  - 10.1101/gr.1949704 
VL  - 14 
IS  - 5 
UR  - http://genome.cshlp.org/content/14/5/901.abstract 
N2  - Comprehensive genome scans involving many thousands of SNP assays will require significant amounts of genomic DNA from each sample. We report two successful methods for amplifying whole-genomic DNA prior to SNP analysis, multiple displacement amplification, and OmniPlex technology. We determined the coverage of amplification by analyzing a SNP linkage marker set that contained 2320 SNP markers spread across the genome at an average distance of 2.5 cM. We observed a concordance of >99.8% in genotyping results from genomic DNA and amplified DNA, strongly indicating the ability of both methods used to amplify genomic DNA in a highly representative manner. Furthermore, we were able to achieve a SNP call rate of >98% in both genomic and amplified DNA. The combination of whole-genome amplification and comprehensive SNP linkage analysis offers new opportunities for genetic analysis in clinical trials, disease association studies, and archiving of DNA samples. 
ER  -