@article{Bishop01052004,
author = {Bishop, Amy and Fielding, Sue and Dyson, Paul and Herron, Paul}, 
title = {Systematic Insertional Mutagenesis of a Streptomycete Genome: A Link Between Osmoadaptation and Antibiotic Production},
volume = {14}, 
number = {5}, 
pages = {893-900}, 
year = {2004}, 
doi = {10.1101/gr.1710304}, 
abstract ={The model organism Streptomyces coelicolor represents a genus that produces a vast range of bioactive secondary metabolites. We describe a versatile procedure for systematic and comprehensive mutagenesis of the S. coelicolor genome. The high-throughput process relies on in vitro transposon mutagenesis of an ordered cosmid library; mutagenized cosmids with fully characterized insertions are then transferred by intergeneric conjugation into Streptomyces, where gene replacement is selected. The procedure can yield insertions in upward of 90% of genes, and its application to the entire genome is underway. The methodology could be applied to many other organisms that can receive DNA via RK2/RP4-mediated intergeneric conjugation. The system permits introduction of mutations into different genetic backgrounds and qualitative measurement of the expression of disrupted genes as demonstrated in the analysis of a hybrid histidine kinase and response regulator gene pair, osaAB, involved in osmoadaptation in Streptomyces. The independently transcribed response regulator gene, osaB, is essential for osmoadaptation; when grown with supplementary osmolyte, an osaB mutant cannot erect aerial hyphae and produces up to fivefold greater antibiotic yields than the wild-type strain.}, 
URL = {http://genome.cshlp.org/content/14/5/893.abstract}, 
eprint = {http://genome.cshlp.org/content/14/5/893.full.pdf+html}, 
journal = {Genome Research} 
}