RT Journal
A1 Fan, Jian-Bing
A1 Yeakley, Joanne M.
A1 Bibikova, Marina
A1 Chudin, Eugene
A1 Wickham, Eliza
A1 Chen, Jing
A1 Doucet, Dennis
A1 Rigault, Philippe
A1 Zhang, Baohong
A1 Shen, Richard
A1 McBride, Celeste
A1 Li, Hai-Ri
A1 Fu, Xiang-Dong
A1 Oliphant, Arnold
A1 Barker, David L.
A1 Chee, Mark S.
T1 A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices
JF Genome Research 
JO Genome Research 
YR 2004 
FD May 01 
VO 14 
IS 5 
SP 878 
OP 885 
DO 10.1101/gr.2167504 
UL http://genome.cshlp.org/content/14/5/878.abstract 
AB We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using oligonucleotides containing unique address sequences that can hybridize to universal arrays. Cell-specific gene expression profiles were obtained using this assay for hormone-treated cell lines and laser-capture microdissected cancer tissues. Gene expression profiles derived from this assay were consistent with those determined by qRT-PCR. The DASL assay has been automated for use with a bead-based 96-array matrix system. The combined high-throughput assay and readout system is accurate and efficient, and can cost-effectively profile the expression of hundreds of genes in thousands of samples.