TY  - JOUR
A1  - Fan, Jian-Bing
A1  - Yeakley, Joanne M.
A1  - Bibikova, Marina
A1  - Chudin, Eugene
A1  - Wickham, Eliza
A1  - Chen, Jing
A1  - Doucet, Dennis
A1  - Rigault, Philippe
A1  - Zhang, Baohong
A1  - Shen, Richard
A1  - McBride, Celeste
A1  - Li, Hai-Ri
A1  - Fu, Xiang-Dong
A1  - Oliphant, Arnold
A1  - Barker, David L.
A1  - Chee, Mark S.
T1  - A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices
Y1  - 2004/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 878 
EP  - 885 
DO  - 10.1101/gr.2167504 
VL  - 14 
IS  - 5 
UR  - http://genome.cshlp.org/content/14/5/878.abstract 
N2  - We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using oligonucleotides containing unique address sequences that can hybridize to universal arrays. Cell-specific gene expression profiles were obtained using this assay for hormone-treated cell lines and laser-capture microdissected cancer tissues. Gene expression profiles derived from this assay were consistent with those determined by qRT-PCR. The DASL assay has been automated for use with a bead-based 96-array matrix system. The combined high-throughput assay and readout system is accurate and efficient, and can cost-effectively profile the expression of hundreds of genes in thousands of samples. 
ER  -