RT Journal A1 Krzywinski, Martin A1 Wallis, John A1 Gösele, Claudia A1 Bosdet, Ian A1 Chiu, Readman A1 Graves, Tina A1 Hummel, Oliver A1 Layman, Dan A1 Mathewson, Carrie A1 Wye, Natasja A1 Zhu, Baoli A1 Albracht, Derek A1 Asano, Jennifer A1 Barber, Sarah A1 Brown-John, Mabel A1 Chan, Susanna A1 Chand, Steve A1 Cloutier, Alison A1 Davito, Jonathon A1 Fjell, Chris A1 Gaige, Tony A1 Ganten, Detlev A1 Girn, Noreen A1 Guggenheimer, Kurtis A1 Himmelbauer, Heinz A1 Kreitler, Thomas A1 Leach, Stephen A1 Lee, Darlene A1 Lehrach, Hans A1 Mayo, Michael A1 Mead, Kelly A1 Olson, Teika A1 Pandoh, Pawan A1 Prabhu, Anna-Liisa A1 Shin, Heesun A1 Tänzer, Simone A1 Thompson, Jason A1 Tsai, Miranda A1 Walker, Jason A1 Yang, George A1 Sekhon, Mandeep A1 Hillier, LaDeana A1 Zimdahl, Heike A1 Marziali, Andre A1 Osoegawa, Kazutoyo A1 Zhao, Shaying A1 Siddiqui, Asim A1 de Jong, Pieter J. A1 Warren, Wes A1 Mardis, Elaine A1 McPherson, John D. A1 Wilson, Richard A1 Hübner, Norbert A1 Jones, Steven A1 Marra, Marco A1 Schein, Jacqueline T1 Integrated and Sequence-Ordered BAC- and YAC-Based Physical Maps for the Rat Genome JF Genome Research JO Genome Research YR 2004 FD April 01 VO 14 IS 4 SP 766 OP 779 DO 10.1101/gr.2336604 UL http://genome.cshlp.org/content/14/4/766.abstract AB As part of the effort to sequence the genome of Rattus norvegicus, we constructed a physical map comprised of fingerprinted bacterial artificial chromosome (BAC) clones from the CHORI-230 BAC library. These BAC clones provide ∼13-fold redundant coverage of the genome and have been assembled into 376 fingerprint contigs. A yeast artificial chromosome (YAC) map was also constructed and aligned with the BAC map via fingerprinted BAC and P1 artificial chromosome clones (PACs) sharing interspersed repetitive sequence markers with the YAC-based physical map. We have annotated 95% of the fingerprint map clones in contigs with coordinates on the version 3.1 rat genome sequence assembly, using BAC-end sequences and in silico mapping methods. These coordinates have allowed anchoring 358 of the 376 fingerprint map contigs onto the sequence assembly. Of these, 324 contigs are anchored to rat genome sequences localized to chromosomes, and 34 contigs are anchored to unlocalized portions of the rat sequence assembly. The remaining 18 contigs, containing 54 clones, still require placement. The fingerprint map is a high-resolution integrative data resource that provides genome-ordered associations among BAC, YAC, and PAC clones and the assembled sequence of the rat genome.