TY - JOUR A1 - Krzywinski, Martin A1 - Wallis, John A1 - Gösele, Claudia A1 - Bosdet, Ian A1 - Chiu, Readman A1 - Graves, Tina A1 - Hummel, Oliver A1 - Layman, Dan A1 - Mathewson, Carrie A1 - Wye, Natasja A1 - Zhu, Baoli A1 - Albracht, Derek A1 - Asano, Jennifer A1 - Barber, Sarah A1 - Brown-John, Mabel A1 - Chan, Susanna A1 - Chand, Steve A1 - Cloutier, Alison A1 - Davito, Jonathon A1 - Fjell, Chris A1 - Gaige, Tony A1 - Ganten, Detlev A1 - Girn, Noreen A1 - Guggenheimer, Kurtis A1 - Himmelbauer, Heinz A1 - Kreitler, Thomas A1 - Leach, Stephen A1 - Lee, Darlene A1 - Lehrach, Hans A1 - Mayo, Michael A1 - Mead, Kelly A1 - Olson, Teika A1 - Pandoh, Pawan A1 - Prabhu, Anna-Liisa A1 - Shin, Heesun A1 - Tänzer, Simone A1 - Thompson, Jason A1 - Tsai, Miranda A1 - Walker, Jason A1 - Yang, George A1 - Sekhon, Mandeep A1 - Hillier, LaDeana A1 - Zimdahl, Heike A1 - Marziali, Andre A1 - Osoegawa, Kazutoyo A1 - Zhao, Shaying A1 - Siddiqui, Asim A1 - de Jong, Pieter J. A1 - Warren, Wes A1 - Mardis, Elaine A1 - McPherson, John D. A1 - Wilson, Richard A1 - Hübner, Norbert A1 - Jones, Steven A1 - Marra, Marco A1 - Schein, Jacqueline T1 - Integrated and Sequence-Ordered BAC- and YAC-Based Physical Maps for the Rat Genome Y1 - 2004/04/01 JF - Genome Research JO - Genome Research SP - 766 EP - 779 DO - 10.1101/gr.2336604 VL - 14 IS - 4 UR - http://genome.cshlp.org/content/14/4/766.abstract N2 - As part of the effort to sequence the genome of Rattus norvegicus, we constructed a physical map comprised of fingerprinted bacterial artificial chromosome (BAC) clones from the CHORI-230 BAC library. These BAC clones provide ∼13-fold redundant coverage of the genome and have been assembled into 376 fingerprint contigs. A yeast artificial chromosome (YAC) map was also constructed and aligned with the BAC map via fingerprinted BAC and P1 artificial chromosome clones (PACs) sharing interspersed repetitive sequence markers with the YAC-based physical map. We have annotated 95% of the fingerprint map clones in contigs with coordinates on the version 3.1 rat genome sequence assembly, using BAC-end sequences and in silico mapping methods. These coordinates have allowed anchoring 358 of the 376 fingerprint map contigs onto the sequence assembly. Of these, 324 contigs are anchored to rat genome sequences localized to chromosomes, and 34 contigs are anchored to unlocalized portions of the rat sequence assembly. The remaining 18 contigs, containing 54 clones, still require placement. The fingerprint map is a high-resolution integrative data resource that provides genome-ordered associations among BAC, YAC, and PAC clones and the assembled sequence of the rat genome. ER -