@article{Krzywinski01042004, author = {Krzywinski, Martin and Wallis, John and Gösele, Claudia and Bosdet, Ian and Chiu, Readman and Graves, Tina and Hummel, Oliver and Layman, Dan and Mathewson, Carrie and Wye, Natasja and Zhu, Baoli and Albracht, Derek and Asano, Jennifer and Barber, Sarah and Brown-John, Mabel and Chan, Susanna and Chand, Steve and Cloutier, Alison and Davito, Jonathon and Fjell, Chris and Gaige, Tony and Ganten, Detlev and Girn, Noreen and Guggenheimer, Kurtis and Himmelbauer, Heinz and Kreitler, Thomas and Leach, Stephen and Lee, Darlene and Lehrach, Hans and Mayo, Michael and Mead, Kelly and Olson, Teika and Pandoh, Pawan and Prabhu, Anna-Liisa and Shin, Heesun and Tänzer, Simone and Thompson, Jason and Tsai, Miranda and Walker, Jason and Yang, George and Sekhon, Mandeep and Hillier, LaDeana and Zimdahl, Heike and Marziali, Andre and Osoegawa, Kazutoyo and Zhao, Shaying and Siddiqui, Asim and de Jong, Pieter J. and Warren, Wes and Mardis, Elaine and McPherson, John D. and Wilson, Richard and Hübner, Norbert and Jones, Steven and Marra, Marco and Schein, Jacqueline}, title = {Integrated and Sequence-Ordered BAC- and YAC-Based Physical Maps for the Rat Genome}, volume = {14}, number = {4}, pages = {766-779}, year = {2004}, doi = {10.1101/gr.2336604}, abstract ={As part of the effort to sequence the genome of Rattus norvegicus, we constructed a physical map comprised of fingerprinted bacterial artificial chromosome (BAC) clones from the CHORI-230 BAC library. These BAC clones provide ∼13-fold redundant coverage of the genome and have been assembled into 376 fingerprint contigs. A yeast artificial chromosome (YAC) map was also constructed and aligned with the BAC map via fingerprinted BAC and P1 artificial chromosome clones (PACs) sharing interspersed repetitive sequence markers with the YAC-based physical map. We have annotated 95% of the fingerprint map clones in contigs with coordinates on the version 3.1 rat genome sequence assembly, using BAC-end sequences and in silico mapping methods. These coordinates have allowed anchoring 358 of the 376 fingerprint map contigs onto the sequence assembly. Of these, 324 contigs are anchored to rat genome sequences localized to chromosomes, and 34 contigs are anchored to unlocalized portions of the rat sequence assembly. The remaining 18 contigs, containing 54 clones, still require placement. The fingerprint map is a high-resolution integrative data resource that provides genome-ordered associations among BAC, YAC, and PAC clones and the assembled sequence of the rat genome.}, URL = {http://genome.cshlp.org/content/14/4/766.abstract}, eprint = {http://genome.cshlp.org/content/14/4/766.full.pdf+html}, journal = {Genome Research} }